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KMID : 0364820210570020099
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Korean Journal of Microbiology
2021 Volume.57 No. 2 p.99 ~ p.105
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Prevalence of Nitrosopumilus spindle-shaped viruses in different ecogeographical locations
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Gazi Khaled S.
Kim Jong-Geol
Olufowora Olorunleke Y.
Lee Ui-Ju
Rhee Sung-Keun
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Abstract
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Nitrosopumilus spindle-shaped viruses (NSVs) infect ammoniaoxidizingarchaea (AOA). In this study, we detected the presenceof NSVs in different ecosystems using semi-nested PCR of theNSV-specific protein-primed DNA polymerase B (pPolB) gene.
From most samples (75%), NSV-like pPolB genes were amplified,indicating NSVs’ ubiquity in oceans, coastal waters, and terrestrialenvironments. Library analysis of the PCR amplicons showedlow diversity of NSV-like pPolB genes in the ecosystems. Only 20OTUs could be recovered, with 97% nucleotide similarity cutoffsand some dominant OTUs shared over different environments.
Collectively, our data indicate that NSVs might be widespreadin environments where AOA inhabit.
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KEYWORD
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ammonia-oxidizing-archaea, distribution, nitrification, Nitrosopumilus spindle-shaped virus, protein-primed- DNA polymerase
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